Development of RT-PCR Using External and Internal Positive Controls Based on 5' Untranslated Region (UTR) for Molecular Detection of Avian Infectious Bronchitis Virus

development of rt-pcr using external and internal positive controls based on 5' untranslated region (utr) for molecular detection of avian infectious bronchitis virus

infectious bronchitis virus (ibv) belongs to the group of gamma coronaviruses along with other avian coronaviruses. the disease caused by ibv can appear similar to infectious laryngotracheitis, avian influenza, and velogenic newcastle disease, which are high priority diseases. the clinical signs can be accompanied by mortalities in broiler chickens and reduced eggshell and albumin quality in la...

Molecular detection of infectious bronchitis and Newcastle disease viruses in broiler chickens with respiratory signs using Duplex RT-PCR

Infectious bronchitis (IB) and Newcastle disease (ND) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. In the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-RT-PCR. RNA was extracted...

detection of avian infectious bronchitis virus and massachusetts serotype by rt-pcr

the aim of the present study was using rt-pcr for the diagnosis of avian infectious bronchitis virus and massachusetts serotype in tissue samples. optimization of a molecular diagnostic method for the detection of avian bronchitis virus and identification of massachusetts serotype was investigated. in order to detect infectious bronchitis virus (ibv) in tissue samples, an rt-pcr was optimized. ...

Field Study of Infectious Bronchitis Virus in Broiler Using Type-Specific RT-PCR

Detection of Avian Encephalomyelitis Virus in Broiler Chickens in Iran Using RT-PCR and Histopathological Methods

Avian encephalomyelitis is caused by a Tremovirus and primarily affects chickens. The virus can infect young chicks and cause nervous symptoms. Vaccines are used to control the disease in breeders. Recently, the occurrence of the disease is causing concern in the poultry industry. In the present study, we report a case of avian encephalomyelitis in one broiler farm in Kashan (center of Iran). T...

Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR

Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequ...